The text of Ana’s last letter to her husband leaves no doubt as to how much their relationship had deteriorated:
“Sir, your graces displeasure, and my imprisonment, are things so strange onto me, as what to write, or what to excuse, I am altogether ignorant. Towards your good grace, ever cast so foul a blot on your most dutiful wife, and the infinite princess, your daughter; thyne, good King, but let me have a fair trial, and let not my sworn enemies sit as my accusers and judges, yea, let me receive as open trial, for my truth shall fear no open shame (…).
If ever I have found in your sight, if ever the name of Anne Boleyn had been pleasing in your hears, then let me obtain this request (…) From my doleful prison in the Tower, this sixth of May. Your most loyal and ever faithful wife Anne Boleyn”.
“Lord, Your Grace’s displeasure and my imprisonment are as foreign to me as my total ignorance of what I must write to You or for which I apologize. May your good grace cast such a filthy stain forever on your most obedient wife, and on the infinite princess, your daughter. [Isabel I] (…) good King, let me have a fair trial, and let not my mortal enemies act as my accusers and judges, yea, let me receive an open trial, for my truth fears no public shame (…) .
If ever I pleased your eyes, if ever the name of Anne Boleyn was pleasant to your ears, grant me this request (…) From my painful imprisonment in the Tower, this 6th of May. Your most loyal and ever faithful wife.
The request was not granted. The king’s greatest love had turned to hate, and he would endeavor to erase Anne Boleyn’s memory, even destroying Holbein’s portrait of her. Their daughter, years later, would often evoke her mother, use her iconography and jewelry and bring her distant maternal relatives back to court.